Effect of Neuromuscular Joint Facilitation Ankle Joint Pattern on Balance in Healthy Young Men / 中国康复理论与实践

Objective:To compare the effects of neuromuscular joint facilitation (NJF) or isotonic muscle training on dynamic and static balance in healthy young men. Methods:October, 2019, twelve students of Capital Medical University School of Rehabilitation Medicine (aged 20 to 29) were evaluated the balance indexes of single leg standing on stable support with eyes closing and single leg standing on unstable support with eyes opening with BIODEX Balance Tester, and measured 10-meter walking time and Timed 'Up and Go' Test (TUGT) after non-intervention, NJF ankle joint pattern and ankle flexion and extension. Results:The balance indexes, 10-meter walking time and TUGT were the least after NJF (F > 18.941, P < 0.01). Conclusion:NJF ankle joint model can improve the dynamic and static balance ability of human body more effectively than isotonic contraction training alone.

Effect of Kinesio Taping at Knee on Position Sense and Lower-limb Function / 中国康复理论与实践

Objective:To observe the effects of Kinesio Taping and kneepad on position sense of knee in healthy young adult male. Methods:In September, 2019, 20 healthy adult male aged 21 to 27 were selected. They were measured the joint angle error in angle reproduction test, and tested with functional reach test (FRT), 10-meter walking time (10MWT), and Timed "Up and Go" Test (TUGT) under follow four conditions: non-intervention, simple Kinesio Taping, simple kneepad, and both Kinesio Taping and kneepad. Results:For the angle error, it was the most under non-intervention, and the least under simple Kinesio Taping (F = 61.260, P < 0.001). For FRT, there was no significant difference among all the conditions (F = 1.793, P = 0.988). For 10MWT, it was the least under simple Kinesio Taping (F = 23.817, P < 0.001). For TUGT, it was the most under non-intervention (F = 19.865, P < 0.001). Conclusion:Kinesio Taping can improve position sense of knee, as well as walking, without further benefit along with kneepad.

Potentiation and mechanism of cisplatin-induced apoptosis by lidamycin in human hepatoma BEL-7402 cells / 药学学报

<p><b>AIM</b>To investigate the synergetic effect and the mechanism of antitumor action of the antibiotic lidamycin in combination with cisplatin in vitro.</p><p><b>METHODS</b>Cytotoxicity of the drugs was measured by clonogenic assay. Chromatin condensation was observed by co-staining with fluorescent dyes, Hoechst 33342 and propidium iodide. Apoptotic sub-G1 was detected by flow cytometry and DNA ladder was observed using agarose gel electrophoresis. Bcl-2 protein level was detected by Western blot assay.</p><p><b>RESULTS</b>By using clonogenic assay, lidamycin in combination with cisplatin was found to have synergetic effects on the proliferation of human hepatoma BEL-7402 cells. The data showed that BEL-7402 cells treated with cisplatin and lidamycin in combination produced internucleosomal DNA fragmentation analysed by agarose gel electrophoresis. The results of flow cytometry showed that cisplatin and lidamycin administrated in combination showed no obvious change in G1 phase distribution compared with single treatment. However, this combination reduced the S phase arrest and reversed the reduction of G2/M phase induced by single treatment. The results also showed that there was 11.3% or 9.37% of cells undergoing apoptosis in BEL-7402 cells treated with cisplatin or lidamycin, respectively, while it showed 32.4% of apoptotic cells in combination treatment. Cisplatin, lidamycin and combination of cisplatin and lidamycin was shown to induce typical chromatin condensation in BEL-7402 cells. The study showed that 0.5 mumol.L-1 cisplatin or 1 x 10(-4) mumol.L-1 lidamycin alone decreased Bcl-2 protein level, while lidamycin in combination with cisplatin strongly inhibited expression of Bcl-2 proteins in BEL-7402 cells.</p><p><b>CONCLUSION</b>The results suggest that lidamycin enhancement of cisplatin-induced apoptosis associates with decrease of Bcl-2 protein expression, which may be useful for cancer chemotherapy.</p>

Molecular cloning,nucleotides sequence and transient expression of S and M genome segment of hantavirus strain 84Fli / 中华实验和临床病毒学杂志

<p><b>BACKGROUND</b>To sequence, analyze and express the nucleotide sequences of S and M segments of hantavirus strain 84Fli.</p><p><b>METHODS</b>S and M segments of hantavirus 84Fli strain were amplified by RT-PCR, the PCR products were cloned into plasmid pCR2.1-TOPOr. Three clones of each segment which have been sequenced were randomly selected. The coding region of S and M segments were amplified by PCR, and cloned into expressing vector pcDNA3.0. Transient expression of nucleocapsid protein, G1 and G2 glycoproteins in COS7 cells were performed by Lipofectin transfection. The expression of NP, G1 and G2 have been conformed by using immunofluorescence, Western blot and immuno-precipitation methods.</p><p><b>RESULTS</b>The full length S and M segments cDNA have been amplified and cloned. The S and M segments of hantavirus strain 84Fli contained 1 688 and 3 616 nucleotides respectively.</p><p><b>CONCLUSIONS</b>Deduced amino acid sequences of NP and glycoproteins revealed high homologue to other Hantaan viruses. NP, G1 and G2 proteins of 84Fli can be transiently expressed in COS7 cells.</p>